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Загальна кількість знайдених документів : 3 Представлено документи з 1 до 3 |
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| 1. | 
Roomi M. W. Inhibition of growth and expression of inflammation mediators in human leukemic cell line U-937 by a nutrient mixture [Електронний ресурс] / M. W. Roomi, T. Kalinovsky, N. W. Roomi, M. Rath, A. Niedzwiecki // Experimental oncology. - 2013. - Vol. 35, № 3. - С. 180-186. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2013_35_3_7 Aim - a nutrient mixture (NM) containing ascorbic acid, lysine, proline and green tea extract has exhibited anticancer activity in vitro and in vivo in a number of cancer cell lines. We investigated the effect of NM on human leukemic myeloid U-937 cells in vitro by measuring: cell proliferation, MMP expression, invasion, apoptosis, and COX-2 and COX-1 protein expression. Human leukemic cell line U-937 (ATCC) was cultured in RPMI medium supplemented with fetal bovine serum and antibiotics. After 24 h, the cells were treated with NM at 0, 50, 100, 250, 500 and 1000 mu g/ml, in triplicate at each dose. Phorbol 12-myristate 13-acetate (PMA), 100 mu g/ml was added to cells to induce MMP-9 secretion. Cell proliferation was evaluated by MIT assay, MMP expression by gelatinase zymography, invasion through Matrigel, apoptosis by using live green caspase detection kit (Molecular Probe), and COX-2 and COX-1 expression by Western blot. NM had no effect on U-937 cell growth at a concentration of 250 mu g/ml and exhibited an antiproliferative effect at 500 mu g/ml concentration. Zymography did not demonstrate MMP-2 or MMP-9 secretion in normal cells; however, PMA strongly induced MMP-9, which was inhibited by NM in a dose-dependent manner. Cell penetration through Matrigel was significantly reduced (by 95 %) at 250 mu g/ml NM and completely blocked at 500 mu g/ml NM. NM induced slight apoptosis at 100 mu g/ml and moderate at 500 and 1000 pg/ml concentration. NM inhibited COX-2 expression in a dose-dependent fashion and had no effect on COX-1 expression. Conclusions: our results suggest that NM has potent inhibitory effects on U-937 cell growth and expression of inflammatory mediators, significant parameters in AML progression. | ||
| 2. |
Roomi M. W. In vitro inhibition of matrix metalloproteinases, invasion and growth of Fanconi anemia human FANCA and FANCC lymphoblasts by nutrient mixture [Електронний ресурс] / M. W. Roomi, B. Bhanap, N. W. Roomi, A. Niedzwiecki, M. Rath // Experimental oncology. - 2014. - Vol. 36, № 3. - С. 212-214. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2014_36_3_14 Aim - Fanconi anemia is a rare genetic disorder with high propensity for development of cancers, such as aplastic anemia, leukemia and head and neck cancers. Collagen digesting matrix metalloproteinase (MMP) enzymes have been implicated in for their role in various malignancies and to promote metastasis. Biological agents, that prevent extracellular matrix digestion by the MMPs, have been shown to be promising therapeutic approaches to cancer. In this study we investigated effects of a nutrient mixture (NM) containing, ascorbic acid, lysine, proline and green tea extract, on human FANCA and FANCC lymphoblasts for viability, MMP secretion and invasion. Human FANCA lymphoblasts GM13022 and HCS536 were challenged with NM at concentration range within 10 - 1000 mu g/ml. Cell toxicity was assessed by Trypan blue dye exclusion test. Invasion was evaluated through Matrigel and gelatinase zymography for MMP activity. NM was toxic in dose dependent mode to HCS536 cells but not to GM13022 cells. GM13022 cells but not HCS536 cells exhibited MMP-9 secretion, which was inhibited by NM. Matrigel invasion was inhibited in HCS536 cells at 100 and 500 mu g/ml by 27 % and 93 %, respectively. In GM13022 cells, the NM showed completely blocked Matrigel invasion at 500 mu g/ml. Conclusion: NM inhibited MMP secretion and Matrigel invasion in FANCA and inhibited invasion and induced toxicity in FANCC lymphoblasts. These results suggest that the NM may have therapeutic potential in Fanconi anemia associated neoplasia. | ||
| 3. |
Roomi M. W. A nutrient mixture inhibits glioblastoma xenograft U-87 MG growth in male nude mice [Електронний ресурс] / M. W. Roomi, T. Kalinovsky, M. Rath, A. Niedzwiecki // Experimental oncology. - 2016. - Vol. 38, № 1. - С. 54-56. - Режим доступу: http://nbuv.gov.ua/UJRN/EOL_2016_38_1_12 Background - brain tumors are highly aggressive tumors characterized by secretions of high levels of matrix metalloproteinase-2 and -9, leading to tumor growth, invasion and metastasis by digesting the basement membrane and extracellular matrix components. We previously demonstrated the effectiveness of a nutrient mixture (NM) containing ascorbic acid, lysine, proline, and green tea extract in vitro: on activity of urokinase plasminogen activator, matrix metalloproteinases and TIMPs in various human glioblastoma (LN-18, T-98G and A-172) cell lines and on glioblastoma A-172 cell proliferation and Matrigel invasion. Aim - our main objective in this study was to investigate the effect of the NM in vivo on human glioblastoma U-87 MG cell line. Athymic male nude mice inoculated with <$E3~cdot~10 sup 6> U-87 MG cells subcutaneously and were fed a regular diet or a regular diet supplemented with 0,5 % NM. Four weeks later, the mice were sacrificed, the tumors were weighed and measured. The samples were studied histologically. NM inhibited tumor weight and tumor burden by 53 % (p = 0,015) and 48 % (p = 0,010), respectively. Conclusions: these results suggest the therapeutic potential of NM as an adjuvant in the treatment of glioblastoma. | ||
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